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goat anti rabbit antibodies for pedf  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc goat anti rabbit antibodies for pedf
    Goat Anti Rabbit Antibodies For Pedf, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 33314 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+rabbit+antibodies+for+pedf/Anti-rabbit+IgG%2C+HRP-linked+Antibody/pm41166891-94-11-21
    Average 99 stars, based on 33314 article reviews
    goat anti rabbit antibodies for pedf - by Bioz Stars, 2026-09
    99/100 stars

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    Incubation:

    Article Title: Suppression of choroidal neovascularization by nonviral gene transfer of pigment epithelium-derived factor.
    Article Snippet: Membranes were blocked with 5 % skim milk in Tris-buffered saline containing 0.1 % Tween-20 (TBST) at room temperature for 2 h. Membranes were incubated overnight at 4 ◦C with rabbit-anti-human PEDF (0.2 μg/mL, catalog no. 500-P263; Invitrogen, Carlsbad, CA), or mouse anti-β-actin (1:5000, catalog #, MA1–140; Invitrogen). .. After washing with TBST, membranes were incubated with horseradish peroxidase-conjugated secondary goat anti-rabbit antibodies for PEDF (1:2000, catalog #, 7074 s; Cell Signaling Technology, Danvers, MA) or goat anti-mouse antibodies for β-actin (1:4000, catalog #, 7076 s; Cell Signaling Technology) at room temperature for 2 h. Membranes were washed with TBST three times, developed using an enhanced chemiluminescence kit (catalog #, 34075; Thermo Fisher Scientific, Waltham, MA), and imaged with a Bio-Rad lab molecular imaging system. .. Tissue lysates were also used to measure PEDF levels with a PEDF ELISA kit (catalog #, ab213815, Abcam, Cambridge, MA) using the manufacturer’s instructions.

    Imaging:

    Article Title: Suppression of choroidal neovascularization by nonviral gene transfer of pigment epithelium-derived factor.
    Article Snippet: Membranes were blocked with 5 % skim milk in Tris-buffered saline containing 0.1 % Tween-20 (TBST) at room temperature for 2 h. Membranes were incubated overnight at 4 ◦C with rabbit-anti-human PEDF (0.2 μg/mL, catalog no. 500-P263; Invitrogen, Carlsbad, CA), or mouse anti-β-actin (1:5000, catalog #, MA1–140; Invitrogen). .. After washing with TBST, membranes were incubated with horseradish peroxidase-conjugated secondary goat anti-rabbit antibodies for PEDF (1:2000, catalog #, 7074 s; Cell Signaling Technology, Danvers, MA) or goat anti-mouse antibodies for β-actin (1:4000, catalog #, 7076 s; Cell Signaling Technology) at room temperature for 2 h. Membranes were washed with TBST three times, developed using an enhanced chemiluminescence kit (catalog #, 34075; Thermo Fisher Scientific, Waltham, MA), and imaged with a Bio-Rad lab molecular imaging system. .. Tissue lysates were also used to measure PEDF levels with a PEDF ELISA kit (catalog #, ab213815, Abcam, Cambridge, MA) using the manufacturer’s instructions.



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    goat anti rabbit antibodies for pedf  (Cell Signaling Technology Inc)
    99
    Cell Signaling Technology Inc goat anti rabbit antibodies for pedf
    Goat Anti Rabbit Antibodies For Pedf, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+rabbit+antibodies+for+pedf/Anti-rabbit+IgG%2C+HRP-linked+Antibody/pm41166891-94-11-21
    Average 99 stars, based on 1 article reviews
    goat anti rabbit antibodies for pedf - by Bioz Stars, 2026-09
    99/100 stars
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